A. A noble project, but I hope you have plenty of money, resources, and facilities to undertake such a project. I say this because it is very difficult to culture most aquatic insects under aquarium conditions. This is a generality, because I don't know which specific insects you are talking about (stream insects, lake insects). Environmental characteristics such as physical (temperature, flow-rate, substratum type, light), chemical (dissolved oxygen, pH, nutrients), and nutritional (seston, algae, invertebrates, organic matter) requirements differ considerably from species to species and all have to be maintained properly if you expect to raise a particular species through its life cycle. For instance, if you are interested in studying a grazing insect, then you will have to furnish the proper substratum for it to live on, sufficient light to insure that its algal food base will grow on the substratum, the correct types and amounts of nutrients to be sure the algae will grow, and the temperature for all of this to work. The easiest way to solve much of this is to have a flow-through system using a natural stream for a water source. That usually provides everything except the substratum to your system.